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As performed for GII.4 VLPs under the same conditions, the log 10 reductions in GII.4 HuNoV binding to OLe + saliva after exposure to heat treatments (50 °C, 60 °C, and 72 °C) for 30 min were determined using the log (C/C 0) (Fig. 6). A sodium periodate treatment was also added as a negative control for all assays (data not shown).

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We report a new norovirus GII.4 variant, GII.4 Hong Kong, with low-level circulation in 4 Eurasia countries since mid-2017. Amino acid substitutions in key residues on the virus capsid associated with the emergence of pandemic noroviruses suggest that GII.4 Hong Kong has the potential to become the next pandemic variant.

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